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KMID : 0352720120360020205
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Journal of Ginseng Research
2012 Volume.36 No. 2 p.205 ~ p.210
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New Method for Simultaneous Quantification of 12 Ginsenosides in Red Ginseng Powder and Extract: In-house Method Validation
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In Gyo
Ahn Nam-Geun
Bae Bong-Seok
Han Sung-Tai
Noh Kil-Bong
Kim Cheon-Suk
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Abstract
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For quality control of components in Korean red ginseng powder and extract, a new method for simultaneous quantifi cation of 12 ginsenosides (Rg1, Re, Rf, Rh1, Rg2[S], Rg2[R], Rb1, Rc, Rb2, Rd, Rg3[S], and Rg3[R]) was studied. Compared to the offi cial method for quantifi cation of marker substances (ginsenosides Rg1 and Rb1), the proposed methods were guaranteed by in-house method validation. Several criteria such as linearity, specifi city, precision and accuracy were evaluated. For red ginseng powder, recovery (averaging 95% to 105%) was calculated, and analysis of variance was carried out to estimate the relative standard deviation (0.20% to 2.12%). For red ginseng extract, the average recovery rate was 90% to 99% and the relative standard deviation was 0.39% to 2.40%. These results indicate that the proposed method could be used in the laboratory for determination of 12 ginsenosides in red ginseng powder and extract. In addition, this method was found to be suitable for quality control of ginseng products and potentially offer time and cost benefits.
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KEYWORD
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Panax ginseng, Red ginseng, Ginsenosides, Method validation, Analysis, HPLC-photo diode array detector,
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